Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

Blog Article

Within the German national monitoring of zoonotic agents, antimicrobial resistance determination also targets carbapenemase-producing (CP) Escherichia coli by selective isolation from food and livestock.In this monitoring in 2019, the CP E.coli 19-AB01133 was here recovered from pork shoulder.

The isolate was assigned to the phylogenetic group B1 and exhibited the multi-locus sequence-type ST5869.Molecular investigations, including whole genome sequencing, of 19-AB01133 revealed that the isolate carried the resistance genes blaVIM-1, blaSHV-5 and blaCMY-13 on a self-transmissible IncA/C2 plasmid.The plasmid g5210t-p90 was closely related to the previously described VIM-1-encoding plasmid S15FP06257_p from E.

coli of pork origin in Belgium.Our results indicate an occasional spread of the blaVIM-1 gene in Enterobacteriaceae of the European pig population.Moreover, the blaVIM-1 located on an IncA/C2 plasmid supports the presumption of a new, probably human source of carbapenemase-producing Enterobacteriaceae (CPE) entering the livestock and food chain sector.